Staff > group members > Dr Alankar Vaidya> resume


Dr Alankar Vaidya

Postdoctoral Research Associate
Room No.: RH # 713
Phone: 718-260-3602
Fax: 718-260-3075
Email:alankarvaidya@yahoo.com


Current Research Projects:

• Develop and implement different enzyme conjugation strategies
  
• Enzyme crosslinking
  
• Enzyme immobilization – physical adsorption and chemical crosslinking include process optimization and scale up of the process
  
• Protease catalyzed peptide synthesis – exploring medium engineering and steps towards optimization of the process.

Education:

• Ph. D.: 2000, Biotechnology, University of Pune, India Thesis - Design, synthesis and evaluation of polymers for affinity based enzyme separations

• M. Tech.: 1996, Biotechnology and Engineering, Indian Institute of Technology, Kharagpur, India. Projects/Thesis Titles - 1) A Study of Alcohol Precipitation and Ultrafiltration of alpha-amylase 2) Molecular and Biochemical Aspects of an Alkalophilic Thermophilic Bacillus SP. (NCIM 59).

• M. Sc.: 1993, Biochemistry, University of Pune, India. Thesis Title- Use of RFLP in assessment of genetic variability among different strains of Xanthomonas Oryzae pv. oryzae

• B. Sc.: 1991, Chemistry, University of Pune, India.

Professional Experiences:

• Postdoctoral Research Associate: January 2002 to March 2004. Department of Biotechnology, Institute of Food Technology, University of Hohenheim, Emil-Wolff Str. 14, D-70599, Stuttgart, Germany. Project Title: New applications of crosslinked imprinted proteins (CLIPs) in industrial biotransformation processes.

• Visiting Postdoctoral Fellow of Institute for transfer of Industrial Technology (ITIT): October 2000 to December 2000. Environmentally degradable polymer research unit, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute of Biological Resources, Tsukuba Central 6, Tsukuba, Ibaraki 305-8566, Japan. Project Title: Development of Stimuli-responsive biodegradable polymers.

Awards:

• The Japan Society for the Promotion of Science (JSPS) Postdoctoral Fellowship for Foreign Researchers: Environmentally degradable polymer research unit, National Institute of Advanced Industrial Science and Technology (AIST), Research Institute of Biological Resources, Tsukuba Central 6, Tsukuba, Ibaraki 305-8566, Japan. Project Title: Development of stimuli responsive biodegradable polymeric materials.

• The Consejo Superior de Investigaciones Cientificas, Spain for the Postdoctoral Fellowship (Award No. Sb2000-0472): Institute of Catalysis. C.S.I.C. Campus Universitario. Cantoblanco (28049) Madrid. Spain. Project Title: Immobilized enzymes in biocatalytic processes.

• Senior Research Fellowship by Council of Scientific and Industrial Research, India: May 1996 – April 2000 and January 2001 – November 2001, Polymer Science and Engineering Unit, Chemical Engineering Department, National Chemical Laboratory, Dr. Homi Bhabha Road, Pashan, Pune 411008, India. Project Title (Ph. D.) : Design, synthesis and evaluation of polymers for affinity based enzyme separations. Research Guide: Dr. R. A. Mashelkar.

• All India Level Graduate Aptitude Technical Examination (GATE) Fellowship: Securing 96.63 percentile. June 1994- December 1995 ( For M.Tech.)

• Late Dr. B.D.Tilak, (Ex-Director, National Chemical Laboratory, India) Fellowship: For standing first in B.Sc. (Chemistry) from S.P.College (1991).

• Member, Society for Molecular Imprinting (SMI), Department of Pure & Applied
Biochemistry, Center for Chemistry and Chemical Engineering, Lund University, P.O.Box 124, S-221 00 Lund, Sweden.

 

List of Publications:

• Communications

1. Extractive cultivation of recombinant Escherichia coli using aqueous two phase systems for production and separation of extracellular xylanase.
N.Kulkarni, A.Vaidya and M.Rao

Biochemical and Biophysical Research Communications 255: 274-278 (1999).

2. Synthesis, characterization and evaluation of new polymers for thermoprecipitation of adenosine.
A.A.Vaidya, Takao Raku and Yutaka Tokiwa
Biotechnology Letters 23: 805-809 (2001).

3. Altering glucose oxidase to oxidise D-galactose through crosslinking of imprinted protein.
Alankar Vaidya, Alexander Borck, Anja Manns and Lutz Fischer
ChemBioChem 5:132-135 (2004).

4. Alkyl esters of methyl vinyl ether - maleic anhydride copolymer as pH responsive supports for trypsin immobilization.
Alankar Vaidya and Mohan Kulkarni
Communicated

• Full Papers

1. Enhancing ligand-protein binding in affinity thermoprecipitation: Elucidation of spacer effects.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
Biotechnology and Bioengineering 64: 418-425 (1999).

2. LCST in poly (N-isopropylacrylamide) copolymers: high resolution proton NMR investigations.
M.V.Deshmukh, A.A.Vaidya, M.G.Kulkarni, P.R.Rajmohanan and S. Ganpathy.
Polymer 41: 7951-7960 (2000).

3. Thermoprecipitation of lysozyme from egg white using copolymers of N-isopropylacrylamide and acidic monomers.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
Journal of Biotechnology 87: 95-107 (2001).

4. Creating a macromolecular receptor by affinity-imprinting.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
Journal of Applied Polymer Science 81: 1075-1083 (2001).

5. Design and evaluation of new ligands for lysozyme recovery by affinity thermoprecipitation.
A.A.Vaidya, B.S.Lele, M.V.Deshmukh and M.G.Kulkarni.
Chemical Engineering Science 56: 5681-5692 (2001).

6. Crosslinked imprinted protein aggregates: New bioimprinting method for glucose oxidase to improve oxidation of D-galactose
Alankar Vaidya, Luuk van Langen and Lutz Fischer
Communicated

Patents

1. Process for the preparation of molecularly imprinted polymers useful for separation of enzymes
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
Indian Patent No. 1199/Del/1999.
US patent granted on 30 Apr. 2002, US patent No. 6,379,599 B1.

2. Process for the preparation of thermoprecipitating affinity polymers.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
Indian Patent No. 1200/Del/1999.
US Patent granted on 16 July 2002, US Patent No. 6,420,487 B1.

3. Thermoprecipitating polymer containing enzyme specific ligands, process for the preparation thereof and use thereof for the separation of enzymes.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
U.S. patent granted on 12 August 2003, US Patent No. 6,605,714.

4. Thermoprecipitating polymer containing enzyme specific ligands, process for the preparation thereof and use thereof for the separation of enzymes.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
U.S. patent granted on 15 March 2005, US Patent No. 6,867,268.

5. An improved process for the separation of lysozyme.
A.A.Vaidya, B.S.Lele, M.G.Kulkarni and R.A.Mashelkar.
Indian Patent No. 1007/Del/2000.

• Book chapter

Chapter 16. Stabilization of new imprint property of glucose oxidase in pure aqueous medium by crosslinked-imprinting approach.
Alankar Vaidya and Lutz Fischer
Immobilization of enzymes and cells : Second edition. Edited by Prof. Jose Guisan, Published by Humana Press.

• Conference Abstracts

1. Extractive cultivation of recombinant Escherichia coli for production of extracellular xylanase using aqueous two-phase system.

N.Kulkarni, A.Vaidya and M.Rao.
Diamond jubilee symposia and 39th annual conference association of microbiologist of India; Mangalore, India. Held on December 5-7, 1998.

2. Proton (1H) NMR study of LCST phenomenon in copolymers of poly (N-
isopropylacrylamide).
M.V.Deshmukh, P.R.Rajamohanan, A.A.Vaidya, M.G.Kulkarni and S.Ganapathy.
Workshop on applications of NMR in petroleum and petrochemical industries and 5th national symposium on magnetic resonance. Indian Institute of Petroleum, Dehradun, India. Held on February 23-26, 1999.

3. Polymer-polymer and polymer-solvent interactions in the LCST behavior of copolymers of poly (N-isopropylacrylamide) studied by 2D NOESY spectroscopy.
M.V.Deshmukh, A.A.Vaidya, P.R.Rajamohanan, M.G.Kulkarni and S. Ganapathy.
National symposium on magnetic resonance and biomolecular structure and function; Tata Institute of Fundamental Research, Mumbai, India. Held on January 17-20, 2000.

4. Immobilisierte imprintete Enzyme und ihre neuen Katalyseeigenschaften.
L. Fischer und A. Vaidya

Bioperspectives congress and industry presentation (DECHEMA) at Rhein-Main-Hallen Wiesbaden, Rheinstr. 20, 65185 Wiesbaden, Germany on May 4-6, 2004.